Reversibility of cholinephosphotransferase in lung microsomes
- 1 August 1986
- Vol. 21 (8) , 498-502
- https://doi.org/10.1007/bf02535636
Abstract
The effect of cytidine 5′-monophosphate (CMP) on the incorporation of cytidine 5′-diphosphate (CDP) [methyl-14C]choline or [1-14C]dipalmitoylglycerol into phosphatidylcholine (PC) catalyzed by rabbit lung microsomal CDP choline:1,2-diacyl-sn-glycerol cholinephosphotransferase (EC 2.7.8.2) was studied. In the presence of 0.85 mM CMP and nonsaturating diacylglycerol concentration, the incorporation of CDP[14C]choline into PC was markedly stimulated, but the incorporation of [14C]dipalmitoylglycerol into PC was inhibited. This was due to the increase of endogenous diacylglycerol generated from microsomal PC by the cholinephosphotransferase reverse reaction. However, the newly synthesized PC was not readily hydrolyzed in the presence of CMP. The results of this study suggest that the endogenous membranous diacylglycerol is utilized more preferentially for PC synthesis than the exogenous diacylglycerol and that the newly synthesized PC could rapidly equilibrate with the endogenous membrane PC pool.This publication has 26 references indexed in Scilit:
- Synthesis of phosphatidylcholine and phosphatidylethanolamine in relation to the concentration of membrane-bound diacylglycerols of rat lung microsomesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- The rate-limiting step in the biosynthesis of phosphatidylcholine by alveolar type ii cells from adult rat lungBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Utilization of disaturated and unsaturated phosphatidylcholine and diacylglycerols by cholinephosphotransferase in rat lung microsomesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- The reverse reaction of cholinephosphotransferase in rat brain microsomes a new pathway for degradation of phosphatidylcholineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Increased synthesis of phosphatidylcholine by rat lung following premature birthBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- Selective utilization of endogenous unsaturated phosphatidylcholines and diacylglycerols by cholinephosphotransferase of mouse lung microsomesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
- Studies on 1,2-diglycerides formed from endogenous lecithins by the back-reaction of rat liver microsomal cdpcholine: 1,2-diacylglycerol cholinephosphotransferaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Utilization of endogenous phospholipids by the backreaction of CDP-choline (-ethanolamine): 1,2-diglyceride choline (ethanolamine)-phosphotransferase in rat liver microsomesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphataseBiochimica et Biophysica Acta, 1960
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959