Recombinant GABAA receptor function and ethanol
- 14 June 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 324 (2) , 140-142
- https://doi.org/10.1016/0014-5793(93)81380-i
Abstract
Different combinations of cloned subunits of the rat brain GABAA receptor were expressed in Xenopus oocytes. Possible effects of ethanol on the expressed GABA-induced chloride current were determined. The consequence of replacing the γ2s subunit by the alternatively spliced variant γ2L was specifically tested on the responsiveness to ethanol. A significant stimulation of the GABA response was only observed at very high concentrations (> 60 mM) of ethanol. No differential response was observed between subunit combinations containing different γ2 subunit splice variants.Keywords
This publication has 21 references indexed in Scilit:
- Benzodiazepine-induced motor impairment linked to point mutation in cerebeilar GABAA receptorNature, 1993
- Ethanol potentiation of GABAA receptors requires phosphorylation of the alternatively spliced variant of the γ2 subunitFEBS Letters, 1992
- Mechanisms that may underlie the behavioural effects of ethanolProgress in Neurobiology, 1991
- Activation of protein kinase C results in down‐modulation of different recombinant GABAA‐channelsFEBS Letters, 1991
- Inverse but not full benzodiazepine agonists modulate recombinant α6β2γ2 GABAA receptors in transfected human embryonic kidney cellsNeuroscience Letters, 1991
- Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor α4 subunitFEBS Letters, 1991
- Ethanol sensitivity of the GABAA receptor expressed in xenopus oocytes requires 8 amino acids contained in the γ2L subunitNeuron, 1991
- Generation of Two Forms of the γ‐Aminobutyric AcidA Receptor γ‐2‐Subunit in Mice by Alternative SplicingJournal of Neurochemistry, 1991
- Functional expression and sites of gene transcription of a novel α subunit of the GABAA receptor in rat brainFEBS Letters, 1990
- Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-familyNature, 1987