Deficiency in Rhabdomyosarcomas of a Factor Required for MyoD Activity and Myogenesis
- 5 March 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 259 (5100) , 1450-1453
- https://doi.org/10.1126/science.8383879
Abstract
Rhabdomyosarcoma cells express the myogenic helix-loop-helix proteins of the MyoD family but do not differentiate into skeletal muscle cells. Gel shift and transient transfection assays revealed that MyoD in the rhabdomyosarcoma cells was capable of binding DNA but was relatively nonfunctional as a transcriptional activator. Heterokaryon formation with fibroblasts resulted in the restoration of transcriptional activation by MyoD and the differentiation of the rhabdomyosarcoma cells into skeletal muscle cells. These results suggest that rhabdomyosarcomas are deficient in a factor required for MyoD activity.Keywords
This publication has 18 references indexed in Scilit:
- Muscle-specific transcriptional activation by MyoD.Genes & Development, 1991
- MyoD and the regulation of myogenesis by helix-loop-helix proteins.Journal of Clinical Investigation, 1991
- The myoD Gene Family: Nodal Point During Specification of the Muscle Cell LineageScience, 1991
- Activation and repression of myogenesis in somatic cell hybrids: Evidence for trans-negative regulation of MyoD in primary fibroblastsCell, 1990
- MyoD family: a paradigm for development?Genes & Development, 1990
- The protein Id: A negative regulator of helix-loop-helix DNA binding proteinsPublished by Elsevier ,1990
- MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancerCell, 1989
- Expression of a single transfected cDNA converts fibroblasts to myoblastsPublished by Elsevier ,1987
- A specific chromosomal abnormality in rhabdomyosarcomaCytogenetic and Genome Research, 1987
- Cytoplasmic activation of human nuclear genes in stable heterocaryonsCell, 1983