Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses
- 1 December 1975
- journal article
- research article
- Published by Springer Nature in Medical Microbiology and Immunology
- Vol. 162 (1) , 23-27
- https://doi.org/10.1007/bf02123574
Abstract
A wide range of influenza B virus strains with various passage histories uniformly formed well-defined clear plaques with high efficiency in cultures of an established line of canine kidney cells (MDCK). PFU titers of the viruses assayed in MDCK exceeded the titers assayedin ovo. With recently isolated strains such as B/Hong Kong/5/72 and Gifu/2/73, the PFU/EID50 ratios were as high as 100 to 400. MDCK cells have been successfully employed for primary isolation of influenza B viruses from throat washings of patients by direct plaquing.This publication has 9 references indexed in Scilit:
- Influenza Virus Plaque Formation in Different Species of Cell MonolayersJournal of General Virology, 1974
- Genetic Recombination for Antigenic Markers of Antigenically Different Strains of Influenza B VirusJournal of Virology, 1974
- Plaque Formation by Influenza Viruses in Monolayers of Chicken Kidney CellsJournal of General Virology, 1968
- Plaque formation with influenza viruses in explants of chick fibroblasts from embryos of different agesArchiv für die gesamte Virusforschung, 1967
- A sensitive plaque assay for influenza virusesVirology, 1963