Labeling of monodisperse polystyrene microspheres with tightly bound 51Cr
Open Access
- 1 July 1978
- journal article
- research article
- Published by Taylor & Francis in Aihaj Journal
- Vol. 39 (7) , 570-575
- https://doi.org/10.1080/0002889778507810
Abstract
Monodisperse radioactive polystyrene latex microspheres have been prepared by attachment of chromium-51 to latex spheres. Labeling was by emulsion polymerization of chromium acetylacetonate dissolved in styrene monomer using commercially available microspheres. This was accomplished with two successive polymerization steps: radiation excitation and radical polymerization initiated with potassium persulfate. After attachment of the label the particle suspension was purified by repeated centrifugation wash cycles to remove labile radioactivity. Results indicate a radioactive binding yield of greater than 80% to the particles. The stability of the label was tested in in vivo and in vitro leaching studies. In these tests, the activity leaching rate was estimated to be less than 0.2% per day.This publication has 0 references indexed in Scilit: