Cyclic Amp Response of Isolated Snell Adrenocortical Carcinoma 494 Cells to Trophic Hormones and Other Substances
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 2 (6-7) , 403-417
- https://doi.org/10.1080/07435807509084163
Abstract
Suspensions of viable cells were prepared from solid tumor of the Snell adrenocortical carcinoma 494 without the use of proteolytic enzymes. Cyclic AMP formation in these cells was stimulated by ACTH, LH, FSH and TSH but not by prostaglandins (E1 E2, F1α and F2α), insulin and secretin. Glucagon tested at a single dose level of 50 μg increased cyclic AMP to about 65% of the maximum amounts obtained with ACTH. When Ca++ was omitted from the incubation medium, the response to ACTH was considerably reduced while that to LH was essentially unchanged. Low concentrations of EGTA (0.3 mM) abolished the ACTH response almost completely but caused only a partial reduction in the response to LH; as much as 10 mM EGTA was required to obtain complete inhibition of the latter.Keywords
This publication has 23 references indexed in Scilit:
- Stimulation of cyclic adenosine 3′:5′-monophosphate and corticosterone formation in isolated rat adrenal cells by cholera ente rotoxin comparison with the effects of ACTHBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- Adrenocorticotropin stimulation of cyclic adenosine 3′,5′-monophosphate formation in isolated rat adrenal cells the role of membrane sialic acidBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- ULTRASTRUCTURE, STEROIDOGENIC POTENTIAL, AND ENERGY METABOLISM OF THE SNELL ADRENOCORTICAL CARCINOMA 494The Journal of cell biology, 1974
- Metabolic regulation of steroidogenesis in isolated adrenal and adrenocortical carcinoma cells of rat. Effect of adrenocorticotropic hormone and adenosine cyclic 3′: 5′‐monophosphate on the plasma membraneFEBS Letters, 1974
- Metabolic Regulation of Steroidogenesis in Adrenocortical Carcinoma Cells of RatEuropean Journal of Biochemistry, 1973
- CORTICOSTEROIDOGENESIS IN ISOLATED ADRENAL CELLS: EFFECT OF ADRENOCORTICOTROPHIC HORMONE, ADENOSINE 3′,5′-MONOPHOSPHATE AND β1–24ADRENOCORTICOTROPHIC HORMONE DIAZOTIZED TO POLYACRYLAMIDEJournal of Endocrinology, 1972
- Kinetics and dose—response characteristics of adenosine 3′,5′‐monophosphate production by isolated rat adrenal cells stimulated with adrenocorticotrophic hormoneFEBS Letters, 1972
- Isolated Adrenal Cells: Adrenocorticotropic Hormone, Calcium, Steroidogenesis, and Cyclic Adenosine MonophosphateScience, 1972
- Abnormal hormone responses of an adrenocortical cancer adenyl cyclaseJournal of Clinical Investigation, 1971
- Abnormal regulation of adenosine 3′,5′-monophosphate and corticosterone formation in an adrenocortical carcinomaJournal of Clinical Investigation, 1969