NMR Probes of Molecular Dynamics: Overview and Comparison with Other Techniques
Top Cited Papers
- 1 June 2001
- journal article
- review article
- Published by Annual Reviews in Annual Review of Biophysics
- Vol. 30 (1) , 129-155
- https://doi.org/10.1146/annurev.biophys.30.1.129
Abstract
NMR spin relaxation spectroscopy is a powerful approach for characterizing intramolecular and overall rotational motions in proteins. This review describes experimental methods for measuring laboratory frame spin relaxation rate constants by high-resolution solution-state NMR spectroscopy, together with theoretical approaches for interpreting spin relaxation data in order to quantify protein conformational dynamics on picosecond-nanosecond time scales. Recent applications of these techniques to proteins are surveyed, and investigations of the contribution of conformational chain entropy to protein function are highlighted. Insights into the dynamical properties of proteins obtained from NMR spin relaxation spectroscopy are compared with results derived from other experimental and theoretical techniques.Keywords
This publication has 150 references indexed in Scilit:
- Structure-fluorescence correlations in a single tryptophan mutant of carp parvalbumin: solution structure, backbone and side-chain dynamicsJournal of Molecular Biology, 2000
- Relationships between protein structure and dynamics from a database of NMR-derived backbone order parametersJournal of Molecular Biology, 2000
- Effects of Dynamics and Environment on 15N Chemical Shielding Anisotropy in Proteins. A Combination of Density Functional Theory, Molecular Dynamics Simulation, and NMR RelaxationJournal of the American Chemical Society, 1999
- Temperature dependence of intramolecular dynamics of the basic leucine zipper of GCN4: implications for the entropy of association with DNAJournal of Molecular Biology, 1999
- Longitudinal and Transverse 1H−15N Dipolar/15N Chemical Shift Anisotropy Relaxation Interference: Unambiguous Determination of Rotational Diffusion Tensors and Chemical Exchange Effects in Biological MacromoleculesJournal of the American Chemical Society, 1998
- Solution structure and dynamics of linked cell attachment modules of mouse fibronectin containing the RGD and synergy regions: comparison with the human fibronectin crystal structureJournal of Molecular Biology, 1998
- Contributions to protein entropy and heat capacity from bond vector motions measured by NMR spin relaxationJournal of Molecular Biology, 1997
- Protein Backbone Dynamics and 15N Chemical Shift Anisotropy from Quantitative Measurement of Relaxation Interference EffectsJournal of the American Chemical Society, 1996
- Backbone Dynamics of Ribonuclease HI: Correlations with Structure and Function in an Active EnzymeJournal of Molecular Biology, 1995
- Proton-detected NMR relaxation of methylene carbons via stereoselective and random fractional deuterationJournal of the American Chemical Society, 1993