Cultivation of human immunodeficiency virus from whole blood: Effect of anticoagulant and inoculum size on virus growth
- 1 June 1990
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 31 (2) , 161-164
- https://doi.org/10.1002/jmv.1890310215
Abstract
Human immunodeficiency virus (HIV) was cultivated directly from whole blood treated with anticoagulant by cocultivation with phytohaemagglutinin‐stimulated cord blood lymphocytes. When heparin was used as the anticoagulant, isolation rates were low (10% to 56%, depending on the patient group); but when EDTA was used, isolation rates were much higher (50% to 100%). Culture of whole blood gave results identical to those of culture of separated peripheral mononuclear cells, and in some cases virus could be isolated from as little as 10 μl of unseparated EDTA anticoagulated blood.Keywords
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