Auxin Requirements of Sycamore Cells in Suspension Culture

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Abstract
Sycamore (A. pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-D in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this auxin requirement, the cells synthesize IAA. Changes in free 2,4-D and IAA in the cells during a culture passage were monitored. There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 ng/106 cells) on day 2 followed by a decline to 45 ng/106 cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 ng/106 cells) rises slowly to a peak of 1.4 ng/106 cells by day 9 then decreases rapidly to 0.2 ng/106 cells by day 15 (early declining phase) and 0.08 ng/106 cells by day 23 (early stationary phase).