Inactivation of cytosolic aldehyde dehydrogenase viaS‐nitrosylation in ethanol‐exposed rat liver
- 25 July 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (21) , 3967-3972
- https://doi.org/10.1016/j.febslet.2007.07.037
Abstract
Aldehyde dehydrogenase (ALDH) isozymes are critically important in the metabolism of acetaldehyde, thus preventing its accumulation after ethanol‐exposure. We previously reported that mitochondrial ALDH2 could be inactivated viaS‐nitrosylation in ethanol‐exposed rats. This study was aimed at investigating whether cytosolic ALDH1, with a relatively‐low‐Kmvalue (11–18 μM) for acetaldehyde, could be also inhibited in ethanol‐exposed rats. Chronic or binge ethanol‐exposure significantly decreased ALDH1 activity, which was restored by addition of dithiothreitol. Immunoblot analysis with the anti‐S‐nitroso‐Cys antibody showed one immunoreactive band in the immunoprecipitated ALDH1 only from ethanol‐exposed rats, but not from pair‐fed controls, suggestingS‐nitrosylation of ALDH1. Therefore inactivation of ALDH1 viaS‐nitrosylation can result in accumulation of acetaldehyde upon ethanol‐exposure.Keywords
This publication has 44 references indexed in Scilit:
- Increased formation of hepatic N2-ethylidene-2'-deoxyguanosine DNA adducts in aldehyde dehydrogenase 2-knockout mice treated with ethanolCarcinogenesis: Integrative Cancer Research, 2007
- Susceptibility to inhalation toxicity of acetaldehyde in Aldh2 knockout miceFrontiers in Bioscience-Landmark, 2007
- Increased oxidation and degradation of cytosolic proteins in alcohol‐exposed mouse liver and hepatoma cellsProteomics, 2006
- Inhibition of Human Mitochondrial Aldehyde Dehydrogenase by 4-Hydroxynon-2-enal and 4-Oxonon-2-enalChemical Research in Toxicology, 2005
- Inhibition of mitochondrial aldehyde dehydrogenase by nitric oxide‐mediated S‐nitrosylationFEBS Letters, 2005
- Protein S-nitrosylation: purview and parametersNature Reviews Molecular Cell Biology, 2005
- Cellular Signaling Mechanisms in Alcohol-Induced Liver DamageSeminars in Liver Disease, 2004
- Human liver mitochondrial aldehyde dehydrogenase: Three‐dimensional structure and the restoration of solubility and activity of chimeric formsProtein Science, 1999
- Investigation of the role of polymorphisms at the alcohol and aldehyde dehydrogenase loci in genetic predisposition to alcohol-related end-organ damageHepatology, 1991
- Time course of the carbon tetrachloride-induced decrease in mitochondrial aldehyde dehydrogenase activityToxicology and Applied Pharmacology, 1983