A New Method of Evaluating the Potency of Antineuritic Concentrates

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Abstract
A method is described for estimating the antineuritic potency of water-soluble concentrates. It is based on the uniform production of polyneuritis in rats on a diet in which the antineuritic thermolabile component of the vitamin B complex is the sole limiting factor and the determination of the minimum curative dose, injected intravenously. The method claims specificity, rapidity, and a sufficient degree of accuracy to be a useful and reliable guide in the chemical purification of antineuritic concentrates with a view toward isolating the active principle. Much of the present-day confusion concerning the relative merits of the various chemical procedures employed by biochemists in the purification and isolation of the antineuritic vitamin would be clarified if a uniform reliable method were adopted for the standardization of their potent fractions. A method is described for the preparation of an antineuritic concentrate of considerable potency from dried brewer''s yeast. The method simply involves fractional precipitation of inert material with organic solvents, adsorption and elution of the active substance with different solvents under different pH values. On account of the ease and certainty with which active material can be prepared by this method, it is believed that it might well serve as a starting point for further purification.

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