Specific determination of arylsulfatase A activity
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 42 (1) , 33-35
- https://doi.org/10.1007/bf01975881
Abstract
Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.Keywords
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