Effect of Ultraviolet Irradiation on 30‐S Ribosomal Subunits. Identification of the RNA Region Crosslinked to Protein S7
- 1 February 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 104 (1) , 255-262
- https://doi.org/10.1111/j.1432-1033.1980.tb04423.x
Abstract
The effects of UV irradiation on Escherichia coli 30-S ribosomal subunits were studied. At the present doses of radiation (0-4.5 .times. 105 quanta/30-S subunit), only protein S7 was significantly crosslinked to the 16-S RNA. In conditions where 25% of the protein was covalently crosslinked, the ability of the irradiated 30-S subunits to reassociate with 50-S subunits and their activity in polyphenylalanine synthesis decreased strongly. Similar results were obtained by irradiation with a germicide lamp (254 nm) or with a monochromatic UV light at 248 nm. No additional proteins were crosslinked to the 16-S RNA by irradiating 30-S subunits depleted in protein S1 or 70-S ribosomes. The covalent complex of 16-S RNA and protein S7 was isolated and digested by T1 RNase. The oligonucleotide remaining attached to the crosslinked protein was characterized as A-C-C-U-C-G [position 1261-1266]. Analysis of this fragment suggests that protein S7 was linked to the cytosine at position 1265 in the RNA sequence.This publication has 48 references indexed in Scilit:
- Characterization of the regions from E. coli 16 S RNA covalently linked to ribosomal proteins S4 and S20 after ultraviolet irradiationFEBS Letters, 1977
- Specific recognition of guanine bases in protein—nucleic acid complexesFEBS Letters, 1977
- Studies of the binding sites of Escherichia coli ribosomal protein S7 with 16S RNA by ultraviolet irradiationFEBS Letters, 1976
- U.V. induced covalent crosslinking of E.coli ribosomal RNA to specific proteinsBiochemical and Biophysical Research Communications, 1976
- 30S Ribosomal proteins associated with the 3′‐terminus of 16S RNAFEBS Letters, 1975
- Studies of the binding of E. coli ribosomal protein S4 to 16S rRNA after UV irradiation of the S4—16S rRNA complexFEBS Letters, 1975
- Functional heterogeneity of the 30 s ribosomal subunit of Escherichia coli: III. Requirement of protein s1 for translationJournal of Molecular Biology, 1974
- Conversion of non-covalent interactions in nucleoproteins into covalent bonds: UV-induced formation of polynucleotide-protein crosslinks in bacteriophage Sd virionsFEBS Letters, 1974
- A protein-nucleic acid crosslink in 30s ribosomesBiochemical and Biophysical Research Communications, 1973
- Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coliJournal of Molecular Biology, 1967