YopB of Yersinia enterocolitica Is Essential for YopE Translocation
Open Access
- 1 May 2001
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (5) , 3523-3535
- https://doi.org/10.1128/iai.69.5.3516-3518.2001
Abstract
A previous study has shown that YopB of Yersinia spp. is essential for translocation of Yop effectors across the eucaryotic plasma membrane (M.-P. Sory and G. R. Cornelis, Mol. Microbiol. 14:583–594, 1994). However, this role was recently challenged (V. T. Lee and O. Schneewind, Mol. Microbiol. 31:1619–1629, 1999). Using protease protection and digitonin extraction, we reconfirm that YopB of Yersinia enterocolitica is essential for the translocation of YopE into HeLa cell monolayers.Keywords
This publication has 16 references indexed in Scilit:
- ENDOCYTOSIS OF SALMONELLA TYPHIMURIUM 395 MS AND MR10 BY HELA CELLSActa Pathologica Microbiologica Scandinavica Section B Microbiology, 2009
- LcrV is a channel size‐determining component of the Yop effector translocon of YersiniaMolecular Microbiology, 2001
- LcrV, a Substrate for Yersinia enterocolitica Type III Secretion, Is Required for Toxin Targeting into the Cytosol of HeLa CellsPublished by Elsevier ,2000
- LcrQ/YscM1, regulators of the Yersinia yop virulon, are injected into host cells by a chaperone‐dependent mechanismMolecular Microbiology, 2000
- Yersinia enterocolitica TyeA, an Intracellular Regulator of the Type III Machinery, Is Required for Specific Targeting of YopE, YopH, YopM, and YopN into the Cytosol of Eukaryotic CellsJournal of Bacteriology, 2000
- LcrV ofYersinia pestisEnters Infected Eukaryotic Cells by a Virulence Plasmid-Independent MechanismInfection and Immunity, 1999
- Yersinia enterocolitica Type III SecretionPublished by Elsevier ,1999
- Translocation of a hybrid YopE‐adenylate cyclase from Yersinia enterocolitica into HeLa cellsMolecular Microbiology, 1994
- SycE, a chaperone‐like protein of Yersinia enterocolitica involved in the secretion of YopEMolecular Microbiology, 1993
- Comparison of the invasion strategies used by Salmonella cholerae-suis, Shigella flexneri and Yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replicationBiochimie, 1988