Impaired α‐carboxyamidation of gastrin in vitamin C‐deficient guinea pigs
- 3 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (1) , 151-154
- https://doi.org/10.1016/0014-5793(86)80231-9
Abstract
The biosynthetic activation of many hormonal peptides requires enzymatic a‐carboxyamidation via glycine‐extended intermediate forms. By specific radioimmunochemical measurement on extracts of guinea pig antra, we found that vitamin C deficiency induced a 30‐fold increase in glycine‐extended (i.e. inactive) gastrins. The results indicate that ascorbic acid is neccessary for α‐carboxyamidation in vivo, and thus, that the biosynthetic activation of hormonal peptides can be modified by simple dietary means.Keywords
This publication has 14 references indexed in Scilit:
- Carboxypeptidase B-like converting enzyme activity in secretory granules of rat pituitary.Proceedings of the National Academy of Sciences, 1984
- Identification in pituitary tissue of a peptide alpha-amidation activity that acts on glycine-extended peptides and requires molecular oxygen, copper, and ascorbic acid.Proceedings of the National Academy of Sciences, 1983
- Residue-specific radioimmunoanalysis: a novel analytical tool. Application to the C-terminus of CCK/gastrin peptidesJournal of Biochemical and Biophysical Methods, 1983
- Strategies for the biosynthesis of bioactive peptidesTrends in Neurosciences, 1983
- Mechanism of C-terminal amide formation by pituitary enzymesNature, 1982
- Neuropeptide Y—a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptideNature, 1982
- Immunologic evidence that the gene for L-gulono-gamma-lactone oxidase is not expressed in animals subject to scurvy.Proceedings of the National Academy of Sciences, 1976
- Pre - proparathyroid hormone identified by cell - free translation of messenger RNA from hyperplastic human parathyroid tissue.Journal of Clinical Investigation, 1975
- Three components of gastrin in human serum Gel filtration studies on the molecular size of immunoreactive serum gastrinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1972
- Production and Evaluation of Antibodies for the Radioimmunoassay of GastrinScandinavian Journal of Clinical and Laboratory Investigation, 1972