Development of Intestinal Damage after X-Irradiation and H 3 -Thymidine Incorporation into Intestinal Epithelial Cells of Irradiated Goldfish, Carassius auratus, at Different Temperatures

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Abstract
In order to analyze the relation between survival time of irradiated fish and rate of cell renewal in the intestinal epithelium of the fish, mortality of irradiated goldfish (Carassius auratus), histological changes in the intestinal epithelium, and rate of H3-thymidine uptake by epithelial cells after irradiation were studied at different temperatures. When goldfish irradiated with 8 kr of X-rays were kept at 4[degree]C, a majority of the fish survived for more than 110 days. However, between 150 and 200 days after irradiation, about 3/4 of them died. In the cool thermal surroundings, the development of characteristic histological damage of the intestine seldom took place during a period of 100 days after irradiation, but in fish examined at 181 or 201 days marked histological damage had occurred in the intestine, the epithelial cells being increased in size but reduced in number. Autoradiographic studies of intestinal epithelium of fish given an intraperitoneal injection of H3-thymidine showed that at 22[degree]C, the number of labeled cells and of grains /labeled cell reached the maximum within 3 hours after injection of H3-thymidine, whereas at 4*C, the values remained low even at 24 hours. All mitotic figures were labeled at 3 hours after injection at 22 oC, whereas 20 to 80% were labeled at 44 hours at 4oC. H3-thymidine was incorporated into intestinal epithelial cells in both irradiated and non-irradiated fish, but the ratio of labeled mitotic figures was definitely smaller in irradiated fish than in non-irradiated fish at 44 hours after injection at 4[degree]C.

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