MORPHOLOGICAL STUDY OF EARLY PHASES OF PLATELET ADHESION TO FOREIGN SURFACES: EFFECT OF CALCIUMa

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Abstract
The role of calcium in the interfacial reactions occurring during the early phases of blood contact with glass surfaces has been studied using a rheologic technique that permits enumeration of adherent platelets as well as ultrastructural visualization of the blood-glass interface by both transmission and scanning electron microscopy . In the presence of calcium, platelets adhere to the glass surface at a rapid rate. Within 4 minutes, most of the adherent platelets have lost their normal discoid shape and show pseudopod formation and cytoplasmic spreading. These platelets conform with the glass surface and show central apposition of platelet organelles similar to that seen in activated platelets. Fibrin strands and platelet aggregates are also seen. Chelation of calcium using sodium citrate or EDTA results in decreased platelet adhesion or retention to glass. Platelets that are adherent to glass in the presence of citrate and EDTA retain their discoid shape, although an occasional platelet may show pseudopod formation and centralization of organelles. Calcium appears to be an important factor governing the adhesion of platelets to glass and appears to exert this effect on the platelets themselves in their plasma milieu and not on the preceding adsorption of plasma proteins.

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