Problems in the Determination of Erythrocyte Protoporphyrin by Ethyl Acetate-Acetic Acid Extraction
- 1 March 1984
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 21 (2) , 141-145
- https://doi.org/10.1177/000456328402100212
Abstract
Three problems with the ethyl acetate-acetic acid method of protoporphyrin analysis were identified: (1) protoporphyrin extraction from whole blood may yield low recoveries in comparison with extraction from prediluted blood; (2) recovery of protoporphyrin is low with some batches of ethyl acetate, but such unsatisfactory reagent can be identified by an iodide oxidation test; (3) protoporphyrin standards prepared with deionised water may be unstable. However, glass-distilled water is satisfactory.This publication has 2 references indexed in Scilit:
- Micro-Scale Photofluorometric Determination of "Free Erythrocyte Porphyrin" (Protoporphyrin IX)Clinical Chemistry, 1975
- Assays for Porphyrins, δ-Aminolevulinic-Acid Dehydratase, and Porphyrinogen Synthetase in Microliter Samples of Whole Blood: Applications to Metabolic Defects Involving the Heme PathwayProceedings of the National Academy of Sciences, 1972