Immunocytochemical Localization of GnRH Precursor in the Hypothalamus of European Starlings during Sexual Maturationand Photorefractoriness

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Abstract
Immunocytochemistry with quantitative image analysis, for both GnRH and its precursor proGnRH‐GAP, was used in male European starlings (Sturnus vulgaris) to investigate four stages of a photoperiodically‐induced reproductive cycle. Four different groups of birds were examined: photosensitive but sexually immature, sexually mature, undergoing gonadal regression, and after the completion of regression and fully photorefractory.

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