Effects of Temperature and Photoperiod on the Egg Diapause of Labops hesperius Uhler 1 , 2

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Abstract
Labops hesperius Uhler diapauses as an egg that is in an advanced stage of embryogenesis with well-developed head, eyes, and appendages. Eggs required a minimum of 60 days chilling at 3 or 9°C and approximately 2 wk incubation at 15°C to hatch. Exposure to –15°C did not prevent hatching and, in general, the percent hatch was greater when the eggs were gradually acclimated to 3 or –15°C. Eggs did not hatch if the chilling began in August but 40% or more of the eggs hatched when exposure to cold was delayed until September, October, or November. Length and sequence of different photoperiods in both the chill and incubation temperatures also significantly influenced the percent hatch.

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