EBV-TRANSFORMATION OF SURFACE IGA-POSITIVE HUMAN-LYMPHOCYTES

  • 1 January 1980
    • journal article
    • research article
    • Vol. 125  (1) , 194-196
Abstract
Surface Ig[immunoglobulin]A-positive human lymphocytes were selected, separated and immortalized with Epstein-Barr virus (EBV). Selection was carried out by rosetting the cells with ox erythrocytes, coated with rabbit anti-human IgA, followed by separation on Ficoll-Isopaque. Stable monoclonal lines were established by cloning in agarose. EBV-transformation is not restricted to the IgM-positve B [bone marrow-derived] cell category but can be extended to minority classes of B lymphocytes. The method provides the means to establish other minority categories as continuous lines, depending on the efficiency of the pre-selection procedure.