Inositol phospholipid metabolism and myoblast fusion
- 14 July 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 214 (1) , 77-82
- https://doi.org/10.1042/bj2140077
Abstract
The fusion of chick embryonic myoblasts was studied in tissue culture. Myoblasts are maintained at 0.1 .mu.M-Ca2+ for 50 h. During this time they achieve fusion competence. Fusion is initiated by raising the medium Ca2+ concentration to 1.4 mM. A rapid breakdown of the polyphosphoinositides was detected within 3 min of Ca2+ addition. Rapid synthesis of phosphatidic acid was also detected at this time. Breakdown of phosphatidylinositol and synthesis of 1,2-diacylglycerol were also detected. Other phospholipids were unaffected. Sr2+ could replace Ca2+ in this process but Mg2+ could not and also inhibited the Ca2+ effect. The Ca2+-ionophore A23187 stimulated further apparent polyphosphoinositide breakdown in the presence of Ca2+. The results are discussed with respect to myoblast fusion.This publication has 27 references indexed in Scilit:
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