Enzyme-linked immunosorbent assay for determination of HLA: gene dose effect
- 11 December 2008
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 23 (5) , 284-289
- https://doi.org/10.1111/j.1399-0039.1984.tb00047.x
Abstract
Polymorphic HLA antigens could be detected on fresh and dried peripheral blood lymphocytes using the enzyme-linked immunosorbent assay (ELISA) with HLA alloantisera. It was investigated whether the ELISA technique can be used in determination of gene-dose effect for antigens of the HLA-A and B loci. Lymphocytes from HLA-A1 and HLA-B14 homozygous individuals are shown to bind significantly more anti-HLA alloantibodies than their heterozygous siblings for the same HLA antigens. ELISA is a sensitive and reliable technique for the qualitative and quantitative assay of polymorphic HLA determinants.This publication has 7 references indexed in Scilit:
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