Labelling of rat vagal preganglionic neurones by carbocyanine dye DiI applied to the heart
- 1 April 1999
- journal article
- research article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 10 (6) , 1177-1181
- https://doi.org/10.1097/00001756-199904260-00004
Abstract
WE describe a method for applying the carbocyanine dye DiI to the rat heart that takes advantage of the dye's lipophilic properties and its ability to diffuse easily into tissues, and results in specific retrograde labelling of cardiac vagal preganglionic neurones in the medulla oblongata. Most of the labelled neurones were found bilaterally in the nucleus ambiguus (81%), with a few sparsely distributed in the dorsal motor vagal nucleus (6.5%), and in an intermediate area located between these two nuclei (12.5%). We contend that the method of applying DiI crystals to the surface of the heart is a more efficient, accurate and reproducible method of retrograde labelling than the injection of tracers into this very delicate tissue.Keywords
This publication has 10 references indexed in Scilit:
- Barosensitive cardioinhibitory neurons in the medulla: Comparison of FosB/ChAT-positive neurons with CT-HRP-labeled neuronsJournal of the Autonomic Nervous System, 1997
- A reliable and sensitive method for fluorescent photoconversionJournal of Neuroscience Methods, 1996
- Localization of cardiac vagal preganglionic motoneurones in the rat: Immunocytochemical evidence of synaptic inputs containing 5‐hydroxytryptamineJournal of Comparative Neurology, 1993
- Identification and dissociation of cardiovascular neurons from the medulla for patch clamp analysisNeuroscience Letters, 1991
- Dil and DiO: versatile fluorescent dyes for neuronal labelling and pathway tracingTrends in Neurosciences, 1989
- Origins of cardiac vagal preganglionic fibers: A retrograde transport studyBrain Research, 1982
- Medullary origin of vagal preganglionic axons to the heart of the catJournal of the Autonomic Nervous System, 1982
- Horseradish peroxidase localization of cardiac vagal preganglionic somataBrain Research, 1980
- Localization of vagal cardioinhibitory preganglionic neurons within rat brain stemJournal of Comparative Neurology, 1979