gRNA/pre-mRNA annealing and RNA chaperone activities of RBP16
- 25 April 2008
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 14 (6) , 1069-1080
- https://doi.org/10.1261/rna.982908
Abstract
Editing in trypanosomes involves the addition or deletion of uridines at specific sites to produce translatable mitochondrial mRNAs. RBP16 is an accessory factor from Trypanosoma brucei that affects mitochondrial RNA editing in vivo and also stimulates editing in vitro. We report here experiments aimed at elucidating the biochemical activities of RBP16 involved in modulating RNA editing. In vitro RNA annealing assays demonstrate that RBP16 significantly stimulates the annealing of gRNAs to cognate pre-mRNAs. In addition, RBP16 also facilitates hybridization of partially complementary RNAs unrelated to the editing process. The RNA annealing activity of RBP16 is independent of its high-affinity binding to gRNA oligo(U) tails, consistent with the previously reported in vitro editing stimulatory properties of the protein. In vivo studies expressing recombinant RBP16 in mutant Escherichia coli strains demonstrate that RBP16 is an RNA chaperone and that in addition to RNA annealing activity, it contains RNA unwinding activity. Our data suggest that the mechanism by which RBP16 facilitates RNA editing involves its capacity to modulate RNA secondary structure and promote gRNA/pre-mRNA annealing.Keywords
This publication has 51 references indexed in Scilit:
- RNA Editing in Trypanosoma brucei Requires Three Different EditosomesMolecular and Cellular Biology, 2008
- Structure and Function of Cold Shock Proteins in ArchaeaJournal of Bacteriology, 2007
- RNA chaperone activity and RNA-binding properties of the E. coli protein StpANucleic Acids Research, 2007
- Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote the cold adaptation process in Escherichia coliNucleic Acids Research, 2006
- Functional conservation of cold shock domains in bacteria and higher plantsProceedings of the National Academy of Sciences, 2006
- Arginine methylation regulates mitochondrial gene expression in Trypanosoma brucei through multiple effector proteinsRNA, 2006
- RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reactionRNA, 2006
- Compositionally and functionally distinct editosomes in Trypanosoma bruceiRNA, 2006
- The pleiotropic functions of the Y‐box‐binding protein, YB‐1BioEssays, 2003
- The Major Messenger Ribonucleoprotein Particle Protein p50 (YB-1) Promotes Nucleic Acid Strand AnnealingPublished by Elsevier ,2001