Fertility factors in Pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors

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Abstract
The octane plasmid (OCT) in P. putida strains was transferred at low frequency. However, bacteria which newly received this plasmid showed a transient increase in donor ability. Using Octane+ P. putida as the donor, the transfer of most chromosomal markers was independent of OCT transfer, but the mobilization of the octanoate catabolism genes (octanoic and acetate) was dependent on OCT plasmid transfer. The presence of a fertility factor termed FPo was postulated to explain these results. Strains carrying only this fertility factor were obtained from strains carrying OCT and FPo plasmids. Strains in which the OCT plasmid was transferred at high frequencies were also isolated, and chromosome mobilization by OCT and FPo was compared. A different gradient of transmission by OCT and FPo was observed. Chromosome transfer by OCT was dependent on the bacterial recombination system, but the chromosome transfer by FPo was unaffected by the presence of a rec mutation in the donor strain.

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