A CONVENIENT METHOD FOR ASSAY OF DNA SYNTHESIS IN SYNCHRONIZED HUMAN CELL CULTURES
Open Access
- 1 January 1966
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 28 (1) , 139-143
- https://doi.org/10.1083/jcb.28.1.139
Abstract
A simplified method is described by which DNA synthesis and RNA synthesis can be studied in tissue culture systems. The method consists of incubation of the cells with tritiated precursors, sonication of the cells, application of an aliquot of sonicate to a filter paper disc which is washed with cold trichloracetic acid. The wash procedure removes unincorporated precursors and precipitates the macromolecules on the disc. The discs are counted by liquid scintillation spectrometry. Results obtained by use of this method in a study of 5-aminouracil-synchronized human cells in suspension culture are presented. The method is applicable to any study of incorporation of radioactive precursors into macromolecules in tissue culture systems.Keywords
This publication has 2 references indexed in Scilit:
- Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk methodArchives of Biochemistry and Biophysics, 1961
- Thermal Conversion of Nonpriming Deoxyribonucleic Acid to PrimerJournal of Biological Chemistry, 1959