Some Applications of Chiral Liquid Affinity Chromatography Using Bovine Serum Albumin as a Stationary Phase
- 1 June 1984
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 14 (2) , 139-147
- https://doi.org/10.1080/10826068408070620
Abstract
The enantioselectivity exerted by many proteins can be utilized for direct optical resolution in liquid chromatographic processes whereby the protein is used as a stationary phase. Bovine serum albumin (BSA), covalently bound to a suitable support, acts as a chiral discriminator for a variety of racemic organic compounds in aqueous buffers. Columns packed with BSA-silica can be used for determination of enantiomeric composition in aqueous solvents at very low concentrations by HPLC [high performance liquid chromatography]. This technique opens up new possibilities for the preparative isolation of .mu.g amounts of enantiomers and for studies of stereoselectivity and mechanisms in enzymatic and microbial reactions.This publication has 4 references indexed in Scilit:
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