PRODUCTION OF REACTIVE OXYGEN SPECIES AND CHEMILUMINESCENCE BY HUMAN MONOCYTES DURING DIFFERENTIATION AND LYMPHOKINE ACTIVATION IN VITRO

Publisher Website
Google Scholar
Abstract
The zymosan-induced respiratory burst of human monocytes was studied, measuring the generation of reactive oxygen species (ROS) and luminol-dependent chemiluminescence (CL). The monocytes'' ability to generate ROS declined markedly during the 1st 24 h of culture, but regained the original level during the following 3 days. The CL-response declined steadily during the same period. In vitro activation by lymphokines from lymphocytes stimulated with BCG gave a significant increase in ROS-generation; the CL-response was only marginally increased, but activated cells consistently displayed altered kinetics of the CL-response with a more rapid decline from peak CL-values. The production of ROS is apparently not the limiting factor for the magnitude of the CL-response in the present experimental system. The CL-assay may be an uncertain way of quantitating ROS.