Quantitative Determination of Sugars in Fruits by GLC Separation of TMS Derivatives

Abstract
A rapid quantitative method for the determination of sugars in fruits is described. Sugars are converted into trimethylsiJyl derivatives after separating interfering organic acids by precipitation as lead salts. Fructose, glucose, sucrose, and sorbitol are measured by using ∝-D-glucoheptose as a n internal standard. Twenty-eight authentic fruit samples were analyzed. Recovery studies were made anil results compared with those obtained by using the AOAC Munson-Walker general method for reducing sugars.

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