Three-Dimensional Space- and Time-Resolved Fluorescence Spectroscopy

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Abstract
A fluorescence spectroscopic system with both picosecond time resolution and submicrometer three-dimensional space resolution has been developed for elucidating the dynamics of inhomogeneous photophysical/photochemical phenomena. This system is based on a confocal laser scanning fluorescence microscope and a time-correlated single photon counting method. Results on demonstration experiments are shown to specify the performance of the system. Characteristics and limitations of the system are discussed.