ISOLATION OF CHROMOMYCIN-A3 FROM A NEW SUBSPECIES OF STREPTOMYCES

  • 1 January 1975
    • journal article
    • research article
    • Vol. 38  (5) , 369-377
Abstract
A streptomycete contaminant in a marine fungal culture exhibited antibiotic activity against gram-positive bacteria and mycobacteria. The active principle was determined to be a mixture of as many as 12 components which exhibited properties characteristic of the aureolic acid group of antibiotics. Chromatographic comparisons using reference materials revealed the major and 2nd components of the antibiotic mixture to be indistinguishable from chromomycins A3 and A2, respectively. The major component was isolated using a series of column chromatographic and preparative TLC separations. Confirmation of the identity of the major antibiotic as chromomycin A3 was based on elemental analysis, optical rotation, spectral (UV, IR and NMR) characteristics and properties of an acetate derivative.