Plasma Lactic Dehydrogenase-Elevating Agent of Mice: Effect on Levels of Additional Enzymes.

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Abstract
Summary Infection of mice with the lactic dehydrogenase-elevating agent resulted in a 5–10 fold increase of lactic and isocitric dehydrogenases and a 2–3-fold elevation of malic dehydrogenase, glutamic-oxalacetic transaminase, phosphohexose isomerase and glutathione reductase in their plasma. Significant increases in enzyme activities were observed 2 days after infection and achieved maximum levels within 3–4 days. Infection with this agent had no effect on the levels of aldolase, alpha-glycerophosphate and glucose-6-phosphate dehydrogenases, acid and alkaline phosphatases, glutamic-pyruvic transaminase, and leucine amino peptidase. Elevations in enzymes of plasma in mice bearing sarcoma 180 differed both qualitatively and quantitatively from those resulting from infection with the agent. At the terminal stage of tumor development, lactic dehydrogenase was elevated 10-fold, isocitric and malic dehydrogenases, phosphohexose isomerase, aldolase, glutamic-oxalacetic transaminase, and glutathione reductase 2–5-fold. Alkaline phosphatase was decreased, whereas the activities of the other enzymes studied were normal. The level of plasma lactic dehydrogenase in tumor-bearing mice infected with the agent was 3–5 times higher than that expected from the results with either tumor or virus alone. Such synergistic effect of virus and tumor was not observed with the other enzymes.