Routine Titrations of Lansing Virus and Antibody in Mice: Comparison of Methods of Estimating Endpoints.

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Abstract
For several mos., routine titrations in mice were carried out by a single worker of a pool of Lansing virus, and of samples of plasma gamma globulin, placental gamma globulin, and immune monkey serum. Estimates of the ED50''s and their standard errors were calculated for the individual titrations by the probit method. In addition ED50''s were calculated by the KSrber, Reed and Muench, and moving avg. methods. The standard deviation of the titrations was greater than the error of individual assays, suggesting that titrations carried out on different occasions vary more than those performed at the same time. None of the methods mentioned gave widely different estimates of the ED50. In particular, the ED50''s calculated by the KSrber method compared favorably with those obtained by the probit method. The KSrber method is proposed as a suitable alternative to the Reed and Muench procedure for the calculation of ED50 in routine titrations of virus and antiserum.

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