Isa tin Inhibition of Rat Testicular Alkaline Phosphatase
- 1 July 1979
- journal article
- research article
- Published by S. Karger AG in Enzyme
- Vol. 24 (3) , 152-157
- https://doi.org/10.1159/000458647
Abstract
Isatin inhibits rat testicular alkaline phosphatase (E.C. 3.1.3.1) uncompetitively. The hyperbolic curve relating inhibition as a function of substrate concentration; the persistence of inhibition after the tertiary structure of the enzyme was altered by heat denaturation, exposure to urea or papain digestion; the small changes in entropy, free energy and enthalpy in the presence of isatin, and the number of isatin molecules (n = 1.29) combining with 1 molecule of enzyme indicate the non-allosteric nature of inhibition.This publication has 5 references indexed in Scilit:
- RAT TESTICULAR HYALURONIDASE - SUBCELLULAR-LOCALIZATION AND INTERACTION WITH ISATIN1978
- Alkaline phosphataseArchives of Biochemistry and Biophysics, 1967
- Control of isoleucine, valine and leucine biosynthesisBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1964
- The Enzymology of Control by Feedback InhibitionJournal of Biological Chemistry, 1962
- The purification of alkaline phosphatases of animal tissuesBiochemical Journal, 1954