Some Properties of Glutathione Biosynthesis-deficient Mutants of Escherichia coli B

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Abstract
Summary: Mutants of Escherichia coli B that contain essentially no detectable glutathione were isolated. These mutants had a very low activity of γ-glutamylcysteine synthetase or glutathione synthetase. No significant differences in growth in minimal medium were observed between the mutants and the parental strain. The mutants lacking γ-glutamylcysteine synthetase activity were more susceptible to toxic compounds than either the parental strain or a glutathione synthetase-deficient strain. The mutants lacking γ-glutamylcysteine synthetase activity were also susceptible to oxygen.