High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis
Open Access
- 1 June 1985
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 49 (6) , 1547-1549
- https://doi.org/10.1128/aem.49.6.1547-1549.1985
Abstract
By cloning the β-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis , enzyme production was enhanced 50 times. β-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70°C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, β-galactosidase is suitable for application in industrial processes.Keywords
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