Novel disulfonated tetrazolium salt that can be reduced to a water-soluble formazan and its application to the assay of lactate dehydrogenase
- 1 January 1995
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 120 (1) , 113-116
- https://doi.org/10.1039/an9952000113
Abstract
A new tetrazolium salt, 4-[3-(4-iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate, sodium salt, that produces a highly water-soluble formazan dye upon reduction by reduced nicotinamide adenine dinucleotide (NADH) was synthesized. The reduction of the compound by NADH at a neutral pH is fast owing to its small reduction potential. The applicability of the compound to the assay of lactate dehydrogenase is described in comparison with a prevalent tetrazolium salt.Keywords
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