Tumor promoter PMA stimulates the synthesis and secretion of mouse pro-urokinase in MSV-transformed 3T3 cells: this is mediated by an increase in urokinase mRNA content.

Open Access
Publisher Website
Google Scholar
Abstract
In mouse MSV‐3T3 cells the synthesis of the urokinase form of plasminogen activator was increased 10‐fold after addition of the tumor promoter phorbol‐12‐myristate‐13‐acetate (PMA). PMA also stimulated the secretion of the protein into the culture medium, mostly in the form of enzymatically inactive pro‐urokinase. When assayed by injecting RNA into Xenopus laevis oocytes, the concentration of functional urokinase mRNA was found to be 6‐ to 10‐fold higher in the PMA‐treated cells; a similar increase in urokinase mRNA content was measured by hybridisation with a mouse urokinase cDNA probe. Thus, the induction of plasminogen activator by PMA in MSV‐3T3 cells is accounted for by an increased content of urokinase mRNA.

This publication has 41 references indexed in Scilit: