Coitinuous cell lines from embryonic tissues of ticks (Acari: Ixodidae)

Abstract
Six new cell lines were established in continous culture from embryonic tissues of ixodid ticks. Four were fromDermacentor variabilis and two fromD. parumapertus. The cells are mostly fibroblastic and diploid. Mosquito-borne viruses (Chikungunya, O'nyong, yellow fever, and St. Louis encephalitis) as well as tick-borne ones (Langat, Powassan, Colorado tick fever, Kemerovo, and Sawgrass) replicated in certain of these cell lines, but a nonvector-borne flavivirus, Modoc, did not. An undescribed virus fromD. occidentalis ticks, which could not be isolated in Vero cells or newborn mice, was readily isolated in theD. variabilis cell line.