The expression of immunoglobulin determinants on the surface of antigen‐binding lymphoid cells in mice. I. An analysis of light and heavy chain restrictions on individual cells

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Abstract
The expression of immunoglobulin light and heavy chain determinants on the surface of activated mouse spleen cells has been investigated using anti‐immunoglobulin inhibition of antigen‐binding reactions (rosette formation). The results indicate that the majority of binding cells appearing 4 to 7 days after a single injection of sheep erythrocytes have only one light chain type on their surface but simultaneously express more than one class of heavy chain class. As the response proceeds the proportion of heavy chain class “restricted” cells increases. Between days 15 to 30 following antigen injection the majority of binding cells have only one detectable heavy chain class on their surface.Control studies suggest that the appearance of more than one class of immunoglobulin on the surface of these cells is the result of active synthesis by the same individual cells and is not due to a passive absorption phenomenon.Experiments with antisera to non‐immunoglobulin surface antigens indicate that the majority of antigen‐binding cells inhibited by class specific sera are non, or minimally secreting B type lymphoid cells.