Expression and Functional Characterization of a Rat Sulfotransferase (ST1A1) cDNA for Sulfations of Phenolic Substrates in COS-1 Cells
Open Access
- 1 January 1993
- journal article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 61 (2) , 153-156
- https://doi.org/10.1254/jjp.61.153
Abstract
A cDNA (PST-1)isolated from a rat liver cDNA library was expressed in COS-1 cells and found to encode a form of arylsulfotransferase (termed ST1A1), which sulfated p-nitrophenol, α-naphthol, minoxidil, β-estradiol and dopamine, but not dehydroepiandrosterone and cortisol. ST1A1 showed a mobility identical with a major immunodetectable sulfotransferase contained in the livers of male and female rats in Western blots. In addition, ST1A1 mRNA was detected in the liver and extrahepatic tissues by Northern blots.Keywords
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