Studies on the RIF Test for the Detection of an Avian Leukosis Virus

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Abstract
Modifications of the tests described by Rubin for the assay of RIF in serum, culture fluids, and embryos were studied. Those found particularly successful included: the use of liquid medium rather than agar medium for overlay of cell cultures, thereby promoting a more rapid spread of infection within the culture; the use of sparsely seeded cultures which required longer periods to grow into complete monolayers and eliminated the need for intermediate culture transfers; and challenge with RSV [Rous sarcoma virus] without concurrent transfer of the cell culture. In the course of testing these modifications, nearly 1,000 embryos from 47 non-RIF-viremic, RSVA-positive dams were checked for RIF transmission in eggs. Ten hens were found to shed virus in their eggs intermittently. As many as 10-18 consecutive negative embryos were obtained from some of the shedders.

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