Simazine and Atrazine—Suppression of Denitrification

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Abstract
The effectiveness of the s-triazines simazine [2-chloro-6-bis (ethylamino)-s-triazine] and atrazine [2-chloro-6-(ethylamino)-6-(isopropylamino)-s-triazine and nitrapyrin [2-chloro-6-(trichloromethyl)pyridine] in suppressing denitrification was evaluated in laboratory and greenhouse studies. Parameters used to measure suppression of denitrification were N2 and N2O evolution, soil NO3-N, and plant N (% N × dry weight). Simazine and atrazine significantly reduced N2O evolution in laboratory incubation studies, but had no effect on N2 evolution, while nitrapyrin reduced both N2 and N2O evolution. In greenhouse studies, 0.5 mg kg-1 soil of simazine and atrazine, and 2.0 mg kg-1 soil of nitrapyrin increased soil NO3-N and plant N as compared to the control. It is concluded that simazine and atrazine inhibited denitrification under greenhouse and laboratory conditions and, therefore, that increased media NO3-N availability is the most likely explanation why N concentration in the plant tissue was higher.

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