Mucin Histochemistry by Paradoxical Concanavalin A Staining in Experimental Gastric Cancers Induced in Wistar Rats by N-Methyl-N′-nitro-N-nitrosoguanidine or 4-Nitroquinoline 1-Oxide2

Publisher Website
Google Scholar
Abstract
The histochemistry of mucin in experimental gastric cancers Induced in noninbred Wistar rats by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) or 4-nitroquinoline 1-oxide (4-NQO) was analyzed by various techniques including modifications of the concanavalin A-horseradish peroxidase method (paradoxical concanavalin A staining) and high-iron diamine-Alcian blue (pH 2.5) staining. In the digestive tracts of rats of the control group, mucins were found as neutral class II mucins in superficial mucous cells, sialated and/or sulfated class II mucins in foveola surface mucous cells and in goblet cells, neutral class III mucins in mucous neck cells and in Brunner's gland cells, and sialated and/or sulfated class III mucins in cardiac gland cells and in pyloric gland cells. In noncancerous epithelium of the digestive tracts of rats treated with MNNG or 4-NQO, the reactivities of class II and class III mucins in these cells were normal, but the acidities of the different classes of mucin were increased or decreased. Intestinal metaplasia was rarely found in pyloric mucosae of control rats or rats treated with MNNG or 4-NQO. Well-differentiated adenocarcinomas consisted of a mixture of the following four types of cancer cells: surface mucous cells with sulfated and/or sialated class II mucins, pyloric gland cells with sialated and/or sulfated class III mucins, goblet cells with sulfated class II mucins, and Intestinal absorptive cells with a striated cell border. Occasionally, tumor cells of all types were present in the same tubule. Signet-ring cell carcinomas, mucinous adenocarcinomas, and poorly differentiated adenocarcinomas also consisted of cells containing sulfated and/or sialated class II mucins or sialated and/or sulfated class III mucins.

This publication has 2 references indexed in Scilit: