Abundance and Location of the Small Heat Shock Proteins HSP25 and αB-Crystallin in Rat and Human Heart
- 18 November 1997
- journal article
- research article
- Published by Wolters Kluwer Health in Circulation
- Vol. 96 (10) , 3466-3476
- https://doi.org/10.1161/01.cir.96.10.3466
Abstract
Background In the heart, there are high constitutive levels of the two related small heat shock proteins, HSP25 and αB-crystallin. To gain insight into their functional role, we have analyzed abundance and location of both proteins in rat and human hearts at different stages of development and in diseased state. Methods and Results Immunoblotting analysis of rat ventricular tissue at fetal, neonatal, and adult stages reveals the level of HSP25 to decline strongly during development, whereas the level of αB-crystallin remains nearly constant. In parallel, the portion of phosphorylated isoforms of HSP25 decreases as shown by two-dimensional polyacrylamide gel electrophoresis. HSP25 is detected in cardiomyocytes and endothelial and vascular smooth muscle cells, whereas αB-crystallin is detected in cardiomyocytes only by immunofluorescence and immunoelectron microscopy. Both proteins colocalize in the I-band and M-line region of myofibrils in cardiomyocytes. In diseased and transplanted adult human hearts, HS...Keywords
This publication has 50 references indexed in Scilit:
- Variation in Expression of hsp27 Messenger Ribonucleic Acid during the Cycle of the Seminiferous Epithelium and Co-Localization of hsp27 and Microfilaments in Sertoli Cells of the Rat1Biology of Reproduction, 1996
- Transgenic mice expressing the human heat shock protein 70 have improved post-ischemic myocardial recovery.Journal of Clinical Investigation, 1995
- Overexpression of the rat inducible 70-kD heat stress protein in a transgenic mouse increases the resistance of the heart to ischemic injury.Journal of Clinical Investigation, 1995
- Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteinsFEBS Letters, 1992
- The small heat shock protein hsp25 is accumulated in P19 embryonal carcinoma cells and embryonic stem cells of line BLC6 during differentiationDifferentiation, 1992
- HSP27 is a mediator of sustained smooth muscle contraction in response to bombesinBiochemical and Biophysical Research Communications, 1991
- αB subunit of lens-specific protein α-crystallin is present in other ocular and non-ocular tissuesBiochemical and Biophysical Research Communications, 1989
- The phosphorylation sites of the B2 chain of bovine α-crystallinBiochemical and Biophysical Research Communications, 1987
- The Quaternary Structure of Bovine alpha-Crystallin. Size and Charge Microheterogeneity: More than 1000 Different Hybrids?European Journal of Biochemistry, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970