Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity
- 26 February 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 381 (1-2) , 1-6
- https://doi.org/10.1016/0014-5793(96)00033-6
Abstract
The main ligand‐binding determinant of the human urokinase receptor (uPAR) is located in the amino terminal domain D1, but when isolated this domain presents a 1500 fold lower affinity than the intact three‐domain uPAR (D1D2D3) [1]. uPAR mutants missing either domain 2 (D1HD3) or domain 3 (D1D2) were expressed in murine LB6 cells and showed to be properly GPI‐anchored to the cell surface. Binding assays with [125I]ATF demonstrated that these mutants possessed a normal (D1D2) or slightly reduced (D1HD3) affinity, indicating that a high ligand‐affinity may be achieved by a combination of D1 with domain D2 or D3.Keywords
This publication has 22 references indexed in Scilit:
- Biosynthesis and apical localization of the urokinase receptor in polarized MDCK epithelial cellsFEBS Letters, 1995
- Structure—function relationships in the receptor for urokinase‐type plasminogen activator Comparison to other members of the Ly‐6 family and snake venom α‐neurotoxinsFEBS Letters, 1994
- Ligand Interaction between Urokinase-Type Plasminogen Activator and Its Receptor Probed with 8-Anilino-1-naphthalenesulfonate. Evidence for a Hydrophobic Binding Site Exposed Only on the Intact ReceptorBiochemistry, 1994
- Induction of cell migration by pro-urokinase binding to its receptor: possible mechanism for signal transduction in human epithelial cells.The Journal of cell biology, 1994
- Structure of a soluble, glycosylated form of the human complement regulatory protein CD59Structure, 1994
- Urokinase-type plasminogen activator and its receptor: new targets for anti-metastatic therapy?Trends in Pharmacological Sciences, 1994
- Colocalization of the Human CD59 Gene to 11p13 with the MIC11 Cell Surface AntigenGenomics, 1993
- Cytokines induce urokinase-dependent adhesion of human myeloid cells. A regulatory role for plasminogen activator inhibitors.Journal of Clinical Investigation, 1993
- Structural requirements for glycosyl‐phosphatidylinositol‐anchor attachment in the cellular receptor for urokinase plasminogen activatorEuropean Journal of Biochemistry, 1992
- Regulation of urokinase receptors in monocytelike U937 cells by phorbol ester phorbol myristate acetate.The Journal of cell biology, 1989