The spectrophotometric determination of protein at 210mμ

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Abstract
Absorption measurements at 210 m[mu] can be usefully employed for protein estimation. At this wavelength the total proteins of 40 normal and pathological sera had a mean specific extinction coefficient E[image] of 20.5 [plus or minus] 0.14. Albumin, [alpha]-, [beta]- and gamma-globulin all had m specific extinction e[image] near 20. The method is directly applicable to all proteins studied except those containing unusually large amounts of aromatic amino acids. A method for the estimation of total serum proteins and for albumin: globulin ratios based on the above is described. Bovine serum albumin had almost the same specific extinction coefficient as serum proteins and formed a useful reference standard. The use of the extinction at 210 m[mu] for the detection of very small quantities of protein (2[mu]g/ml) and its application to column chromatography are discussed. In this connection it is necessary to avoid the use of buffers containing carboxylic acids.