Preparation of Equine Luteinizing Hormone1

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Abstract
A method is described for the preparation of purified equine luteinizing hormone having a specific activity essentially equal to the NIH-LH-Sl when measured by the ovarian ascorbic acid depletion assay. Purification was achieved by extraction of whole glands with potassium chloride, fractionation of the extract with ethanol and trichloroacetic acid, and tandem chromatography on the anion exchanger DEAE-cellulose, and the cation exchanger IRC-50. FSH and TSH contamination was low, and the yield of the purified LH fraction was 367 mg/kg whole glands. (Endocrinology76: 762, 1965)