15N NMR study of a mixture of uniformly labeled tRNAs.
- 1 August 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (16) , 4834-4837
- https://doi.org/10.1073/pnas.79.16.4834
Abstract
15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30.degree. C to 70.degree. C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.This publication has 4 references indexed in Scilit:
- Nitrogen-15 nuclear magnetic resonance spectroscopy of some nucleosides and nucleotidesJournal of the American Chemical Society, 1977
- Applications of natural-abundance nitrogen-15 nuclear magnetic resonance to large biochemically important molecules.Proceedings of the National Academy of Sciences, 1975
- Chapter 4 Preparation of RNA and Ribosomes from YeastPublished by Elsevier ,1975
- ISOTOPICALLY LABELED NUCLEIC ACID FROM YEASTJournal of Biological Chemistry, 1949